MORPHOLOGICAL AND BIOCHEMICAL CHANGES IN THE PROCESS OF HEALING OF BURN WOUNDS WITH OINTMENTS BASED ON CARBOXYMETHYL CHITOSAN APIS MELLIFERA
Keywords:
Chitin, chitosan, bees, carboxymethyl chitosan, hidrofill, alkylation, modification, modeling, burn injury, epithelialization.Abstract
This article presents the results of obtaining biopolymers of carboxymethyl esters of chitosan from a new promising source - dry dead bees. Dried and crushed deadwood, collected during the spring renewal of the bee colony, was used. The process was carried out under general conditions according to the general method of alkylation. Provides information about thermal skin burns and emerging pathophysiological changes in integumentary tissues. The aim of studying and treat these severe processes, burn the skin of animals. A new model of thermal skin injury in rats has been created. For the occurrence of a skin burn, a soldering gun with a flat rod PP REXANT ZD-715 12-0188 with a tip heating temperature of up to 400 0C was used. The degree of the burn was determined by histological examination of skin tissues under a microscope, which was grade IIIA and IIIB. Positive results of using an ointment based on carboxymethylchitosan (CMXZ) have been recorded.
References
Anitha A., Maya S., Deepa N.et al. “Efficient water soluble O-carboxymethyl chitosan nanocarrier for the delivery of curcumin to cancer cells,” Carbohydrate Polymers, vol. 83, no. 2, 2011. Р.452–461.
Laudenslager M. J., Schiffman J. D., Schauer C. L. Carboxymethyl chitosan as a matrix material for platinum, gold, and silver nanoparticles // Biomacromolecules, vol. 9. no. 10, 2008. Р.2682–2685.
An N.T., Thien D.T., Dong N.T., Dung P.L. Watersoluble N-carboxymethylchitosan derivatives: preparation, characteristics and its application //Carbohydrate Polymers, vol. 75, no. 3, 2009. Р.489–497.
Ихтиярова Г.А., Курбанова Ф.Н. Получение экологически чистого биополимера карбоксиметилхитозана из пчеленного подмора APIS MELLIFERA // Международной научно-технической on-line конференции на теме “Проблемы и перспективы инновационной техники и технологий в сфере охраны окружающей среды” 18 сентябрь 2020 г.С.294-296.
Курбанова Ф.Н., Нуритдинова Ф., Хайдарова Х. Способ получения и физико-химические свойства хитина и хитозана из подмора пчел // Развитие науки и технологий. – Бухара. № 4. 2018. С.66-70.
Ихтиярова Г.А., Курбанова Ф.Н., Хазратова Д.А., Турабджанов С.М. Биополимер хитин ва хитозаннинг табиатда тарқалиши. Табиий фанлар соҳасидаги долзарб муаммолар ва инновацион технологиялар. Халқаро илмий-техник on-line анжуман. Тошкент -2020 йил 20-21 ноябр Б.92-94
Кличева О.Б., Рашидова С.Ш. Синтез карбоксиметилированного хитина Вombix mori.- Конференчиѐ молодых ушенных Актуальные проблемы химии природных соединений. Ташкент, 2015.- С.119.
Sattarova D.M. Preparation of Carboxymethyl chitosan nanofibers by electrospinning method // International Journal of Materials and Science, USA. 2019, 9(2). P.29-33.
Summer GJ, Puntillo KA, Miaskowski C, Green PG, Levine JD. Burn injury pain: The continuing challenge. The Journal of Pain. 2007;8(7):533-548. DOI: 10.1016/j.jpain.2007.02.426.
Horton JW. Left ventricular contractile dysfunction is a complication of thermal injury. Shock. 2004;22(6):495-507. DOI: 10.1097/01.shk.0000145205.51682.c3
Ashburn MA. Burn pain: The management of procedure-related pain. The Journal of Burn Care & Rehabilitation. 1995; 16(3 Pt 2):365-371.
Asko-Seljavaara S. Burn research--animal experiments. Acta Physiologica Scandinavica. Supplementum. 1986; 554:209-213.
Abdullahi A, Amini-Nik S, Jeschke M. Animal models in burn research. Cellular and Molecular Life Sciences. 2014. 71(17):3241-3255. DOI: 10.1007/s00018-014-1612-5.
Andrews CJ, Kempf M, Kimble R, et al. Development of a consistent and reproducible porcine scald burn model. PLoS One. 2016.11(9):e0162888. DOI: 10.1371/journal.pone.0162888
Dahiya P. Burns as a model of SIRS. Frontiers in Bioscience. 2009.14.4962-4967. DOI: 10.2741/3580
Курбанова Ф.Н., Ихтиярова Г.А., Джумаева М.К. Способ получения и физико-химические свойства карбоксиметил эфиров хитозана из подмора пчел. universum: технические науки. 3(96) март. Москва-2022. Часть 5. С.18-23.
Kurbonova F.N., Ikhtiyarova G.A. Мethod of obtaining and physico-chemical properties of carboxymethyl ethers of chitosan from bees inanimate. Еurasian journal of academic research volume 2, issue 2 february 2022. Р.467-472.
Khojiev D.Ya., Kurbonova F.N. Сreation of a new model of burns in rats with the determination of their degree and the use of carboxymethylchitosan apis mellifera. Eurasian journal of academic research, https://doi.org/10.5281/zenodo.6778484, Volume 2 Issue 6, June 2022, Р.1115-1120.
